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transblot sd apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad transblot sd apparatus
    Transblot Sd Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transblot+sd+apparatus/Trans-Blot+SD/pmc12870770-214-26-29
    Average 99 stars, based on 3966 article reviews
    transblot sd apparatus - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    SDS Page:

    Article Title: The cytochrome c oxidase subunit COX6B1 is required for redox-sensitive early assembly and late stabilization of complex IV.
    Article Snippet: Samples were separated on a precast commercial native gel (NativePAGE 3-12% Bis-Tris Gels, Thermo Fisher Scientific, Cat#BN1001BOX) as described (71). .. Proteins separated by SDS-PAGE were transferred on polyvinylidene difluoride (PVDF) membranes (Immobilon FL 0,45 μm, Merck) by semi-dry electroblotting (0.8 mA/cm2, 1 hour) using a Transblot SD apparatus (Bio-Rad). .. BN-PAGE gels were transferred to PVDF membranes using Dunn’s carbonate buffer (10 mM NaHCO3, 3 mM Na2CO3), applying a constant voltage of 100 V at 4°C for 1 hour using a Mini Trans-Blot® Cell (BioRad).

    Article Title: The cytochrome c oxidase subunit COX6B1 is required for redox-sensitive early assembly and late stabilization of complex IV
    Article Snippet: Samples were separated on a precast commercial native gel (NativePAGE 3%–12% Bis–Tris Gels; Thermo Fisher Scientific, catalog no.: BN1001BOX) as described ( ). .. Proteins separated by SDS-PAGE were transferred to polyvinylidene difluoride membranes (Immobilon FL 0.45 μm; Merck) by semidry electroblotting (0.8 mA/cm 2 , 1 h) using a Transblot SD apparatus (Bio-Rad). .. BN-PAGE gels were transferred to polyvinylidene difluoride membranes using Dunn’s carbonate buffer (10 mM NaHCO 3 , 3 mM Na2CO 3 ), applying a constant voltage of 100 V at 4 °C for 1 h using a Mini Trans-Blot Cell (Bio-Rad).

    Article Title: Mitochondrial translation is the primary determinant of secondary mitochondrial complex I deficiencies
    Article Snippet: .. Proteins separated by SDS-PAGE or BN-PAGE were transferred on polyvinylidene difluoride (PVDF) membranes (Immobilon FL 0.45 μm, Merck) by semi-dry electroblotting (0.8 mA/cm 2 , 1 h) using a Transblot SD apparatus (Bio-Rad). ..

    Article Title: The mTOR inhibitor rapamycin suppresses trigeminal neuropathic pain and p-MKK4/p-p38 mitogen-activated protein kinase-mediated microglial activation in the trigeminal nucleus caudalis of mice with infraorbital nerve injury
    Article Snippet: The protein concentration of the resulting supernatant was measured using the Lowry protein assay (Bio-Rad, Hercules, CA). .. Protein samples (25 μg) were separated on an SDS-PAGE gel and transferred to a polyvinylidene fluoride (PVDF) membrane using a Transblot SD apparatus (Bio-Rad). .. The membranes were blocked with 5% skim milk at room temperature (RT) for 30 min and incubated at 4°C overnight with primary antibodies, followed by incubation with horseradish peroxidase-conjugated secondary antibodies at RT for 1 h. Primary antibody concentrations were as follows: rabbit anti-phospho-mTOR (1:1000, Cell Signaling Technology, Beverly, MA), rabbit anti-mTOR (1:1000, Cell Signaling), rabbit anti-phospho-S6 (1:2000, Cell Signaling), rabbit anti-S6 (1:2000, Cell Signaling), rabbit anti-phospho-4EBP1 (1:2000, Cell Signaling), rabbit anti-4EBP1 (1:2000, Cell Signaling), rabbit anti-phospho-JNK (p-JNK, 1:1000, Cell Signaling), rabbit anti-JNK (1:1000, Cell Signaling), rabbit anti-phospho-p44/42 MAPK (p-ERK1/2, 1:1000, Cell Signaling), rabbit anti-ERK1/2 (1:1000, Cell Signaling), rabbit anti-phospho-p38 MAPK (p-p38, 1:1000, Cell Signaling), rabbit anti-p38 (1:2000, Cell Signaling), rabbit anti-phospho-MKK3/6 (1:1000, Cell signaling), rabbit anti-MKK3 (1:1000, Cell Signaling), rabbit anti-MKK6 (1:1000, Cell Signaling), rabbit anti-phospho-MKK4 (1:1000, Cell Signaling), and rabbit anti-MKK4 (1:1000, Cell Signaling). β-actin was used as the loading control (1,10,000, Sigma).

    Nucleic Acid Electrophoresis:

    Article Title: Biomineralize Mitochondria in Metal‐Organic Frameworks to Promote Mitochondria Transplantation From Non‐Tumorigenic Cells Into Cancer Cells
    Article Snippet: After the assay, the cell plate was transferred to the Cytation 3 Cell Imaging Reader (BioTek, Winooski, VT, USA) for imaging and analysis with Gen5 software (BioTek), enabling cell count determination for each well. .. Proteins separated by SDS‐PAGE gel electrophoresis were transferred onto a PVDF membrane (Immobilon FL 0.45 μm, Merck, Rahway, USA) using the semi‐dry transfer method in the Transblot SD apparatus (Bio‐Rad). ..

    Article Title: Biomineralize Mitochondria in Metal‐Organic Frameworks to Promote Mitochondria Transplantation From Non‐Tumorigenic Cells Into Cancer Cells
    Article Snippet: After the assay, the cell plate was transferred to the Cytation 3 Cell Imaging Reader (BioTek, Winooski, VT, USA) for imaging and analysis with Gen5 software (BioTek), enabling cell count determination for each well. .. Proteins separated by SDS‐PAGE gel electrophoresis were transferred onto a PVDF membrane (Immobilon FL 0.45 μm, Merck, Rahway, USA) using the semi‐dry transfer method in the Transblot SD apparatus (Bio‐Rad). ..

    Membrane:

    Article Title: Biomineralize Mitochondria in Metal‐Organic Frameworks to Promote Mitochondria Transplantation From Non‐Tumorigenic Cells Into Cancer Cells
    Article Snippet: After the assay, the cell plate was transferred to the Cytation 3 Cell Imaging Reader (BioTek, Winooski, VT, USA) for imaging and analysis with Gen5 software (BioTek), enabling cell count determination for each well. .. Proteins separated by SDS‐PAGE gel electrophoresis were transferred onto a PVDF membrane (Immobilon FL 0.45 μm, Merck, Rahway, USA) using the semi‐dry transfer method in the Transblot SD apparatus (Bio‐Rad). ..

    Article Title: Biomineralize Mitochondria in Metal‐Organic Frameworks to Promote Mitochondria Transplantation From Non‐Tumorigenic Cells Into Cancer Cells
    Article Snippet: After the assay, the cell plate was transferred to the Cytation 3 Cell Imaging Reader (BioTek, Winooski, VT, USA) for imaging and analysis with Gen5 software (BioTek), enabling cell count determination for each well. .. Proteins separated by SDS‐PAGE gel electrophoresis were transferred onto a PVDF membrane (Immobilon FL 0.45 μm, Merck, Rahway, USA) using the semi‐dry transfer method in the Transblot SD apparatus (Bio‐Rad). ..

    Article Title: Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle
    Article Snippet: .. Gels were blotted onto PVDF (polyvinylidene difluoride) membrane (Immobilon FL 0.45 μm, Merck) by semi-dry electro-transfer (1 h at 0.8 mA/cm2) using a Transblot SD apparatus (Bio-Rad). ..

    Article Title: The mTOR inhibitor rapamycin suppresses trigeminal neuropathic pain and p-MKK4/p-p38 mitogen-activated protein kinase-mediated microglial activation in the trigeminal nucleus caudalis of mice with infraorbital nerve injury
    Article Snippet: The protein concentration of the resulting supernatant was measured using the Lowry protein assay (Bio-Rad, Hercules, CA). .. Protein samples (25 μg) were separated on an SDS-PAGE gel and transferred to a polyvinylidene fluoride (PVDF) membrane using a Transblot SD apparatus (Bio-Rad). .. The membranes were blocked with 5% skim milk at room temperature (RT) for 30 min and incubated at 4°C overnight with primary antibodies, followed by incubation with horseradish peroxidase-conjugated secondary antibodies at RT for 1 h. Primary antibody concentrations were as follows: rabbit anti-phospho-mTOR (1:1000, Cell Signaling Technology, Beverly, MA), rabbit anti-mTOR (1:1000, Cell Signaling), rabbit anti-phospho-S6 (1:2000, Cell Signaling), rabbit anti-S6 (1:2000, Cell Signaling), rabbit anti-phospho-4EBP1 (1:2000, Cell Signaling), rabbit anti-4EBP1 (1:2000, Cell Signaling), rabbit anti-phospho-JNK (p-JNK, 1:1000, Cell Signaling), rabbit anti-JNK (1:1000, Cell Signaling), rabbit anti-phospho-p44/42 MAPK (p-ERK1/2, 1:1000, Cell Signaling), rabbit anti-ERK1/2 (1:1000, Cell Signaling), rabbit anti-phospho-p38 MAPK (p-p38, 1:1000, Cell Signaling), rabbit anti-p38 (1:2000, Cell Signaling), rabbit anti-phospho-MKK3/6 (1:1000, Cell signaling), rabbit anti-MKK3 (1:1000, Cell Signaling), rabbit anti-MKK6 (1:1000, Cell Signaling), rabbit anti-phospho-MKK4 (1:1000, Cell Signaling), and rabbit anti-MKK4 (1:1000, Cell Signaling). β-actin was used as the loading control (1,10,000, Sigma).



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